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PE anti-human CD4 Antibody
产品名称：
PE anti-human CD4 Antibody
产品类别：
抗体
产品编号：
300507
产品应用：
300507
[价格]
规格 价格 库存
25tests ￥ 392 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.? This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  11. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  2. Chan S, et al. 2022. Nat Cancer. . PubMed
  3. Miura M, et al. 2019. Wellcome Open Res. 3:105. PubMed
  4. Wang F, et al. 2018. Oncogenesis. 7:41. PubMed
  5. Scott e, et al. 2015. J Immunol. 195: 5561 - 5571. PubMed
  6. Tankou SK, et al. 2018. Ann Neurol. 83:1147. PubMed
  7. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  8. Ho CH, et al. 2021. Arthritis Res Ther. 23:199. PubMed
  9. Schank M, et al. 2020. Cell Death Dis. 1.173611111. PubMed
  10. Schlicher L, et al. 2022. Int J Mol Sci. 23:. PubMed
  11. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  12. Elston L, et al. 2020. Br J Haematol. 188:872. PubMed
  13. Hu W, et al. 2021. Vaccines (Basel). 9:. PubMed
  14. Zhao J, et al. 2021. Front Immunol. 12:658420. PubMed
  15. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  16. Xia Y, et al. 2019. Gastroenterol Res Pract. 2019:5436961. PubMed
  17. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  18. Miller IC, et al. 2021. Nature Biomedical Engineering. :. PubMed
  19. Kuo HH, et al. 2018. Immunity. 48:1183. PubMed
  20. Fu W, et al. 2016. Sci Rep. 6:38162. PubMed
  21. Ziegler CGK, et al. 2021. Cell. 184(18):4713-4733.e22. PubMed
  22. Vabret N, et al. 2022. iScience. 25:104599. PubMed
  23. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  24. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  25. Zeng Y et al. 2019. Immunity. 51(5):930-948 . PubMed
  26. Paul S, et al. 2021. Sci Transl Med. 13:. PubMed
  27. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  28. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  29. Chen J, et al. 2017. Sci Rep. 10.1038/s41598-017-11056-9. PubMed
  30. G?rtner K, et al. 2019. J Extracell Vesicles. 8:1573051. PubMed
  31. Anderson DA, et al. 2022. J Exp Med. 219:. PubMed
  32. Beyer M, et al. 2016. Nat Immunol. 17:593-603. PubMed
  33. Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed
  34. Pons S, et al. 2021. Immun Inflamm Dis. 9:1656. PubMed
  35. Lamsfus Calle C, et al. 2021. Front Immunol. 12:625712. PubMed
  36. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  37. Li B, et al. 2019. Oncogenesis. 8:17. PubMed
  38. Anderson NR, et al. 2019. Cell Adh Migr. 13:163. PubMed
RRID
AB_314075 (BioLegend Cat. No. 300507) AB_314076 (BioLegend Cat. No. 300508) AB_2564152 (BioLegend Cat. No. 300550) AB_2562053 (BioLegend Cat. No. 300539)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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