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FITC anti-human CD4 Antibody
产品名称：
FITC anti-human CD4 Antibody
产品类别：
抗体
产品编号：
300505
产品应用：
300505
[价格]
规格 价格 库存
25tests ￥ 238 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.? This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  11. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Chiu H, et al. 2021. iScience. 24:102748. PubMed
  2. Kim MY, et al. 2021. JCI Insight. 6:. PubMed
  3. Zong D, et al. 2021. BMC Biol. 19:79. PubMed
  4. Wahl S, et al. 2016. Nature. 541:81-86. PubMed
  5. Gong B, et al. 2021. Mol Med Rep. 23:00. PubMed
  6. Rolandelli A, et al. 2017. Sci Rep. 7:40666. PubMed
  7. Choudhry V, et al. 2006. Biochem Biophys Res Commun. 348:1107. PubMed
  8. Goletz C, et al. 2018. Front Immunol. 9:1614. PubMed
  9. Soldi R, et al. 2020. PLoS One. 15:e0235705. PubMed
  10. Panwar B, et al. 2021. Genome Res. 31:659. PubMed
  11. Sun L, et al. 2020. J Diabetes Res. 2020:2583257. PubMed
  12. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  13. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
  14. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  15. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  16. Zhao J, et al. 2021. Front Immunol. 12:658420. PubMed
  17. Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed
  18. Qiu XM, et al. 2020. Reproduction. 251:159. PubMed
  19. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  20. Handono K, et al. 2020. Eur J Dent. 0.961111111. PubMed
  21. Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
  22. Fang F, et al. 2022. JCI Insight. 7:. PubMed
  23. Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed
  24. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  25. Pagel J, et al. 2020. Front Immunol. 11:565257. PubMed
  26. Luo X, et al. 2015. J Biol Chem. 290: 28675 - 28682. PubMed
  27. Yang L, et al. 2020. Genes Dis. 7:128. PubMed
  28. Cheng Y, et al. 2021. Immunity. 54(8):1825-1840.e7. PubMed
  29. Hansen EC et al. 2016. eLife. 5 pii: e18447. PubMed
  30. Han L, et al. 2014. J Biol Chem. 289:25546. PubMed
  31. Mastrogiovanni M, et al. 2022. Sci Adv. 8:eabl5942. PubMed
  32. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  33. Hamilton JR, et al. 2021. Cell Reports. 35(9):109207. PubMed
  34. Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed
  35. Zenaro E, et al. 2009. J Leukoc Biol. 86:1393. PubMed
  36. Kang LJ, et al. 2020. Sci Rep. 10:5603. PubMed
  37. Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
  38. Scherpenisse M, et al. 2021. MBio. 12:. PubMed
  39. Tamai Y, et al. 2013. J Immunol. 190:4382. PubMed
  40. Iio K, et al. 2019. Sci Rep. 9:813. PubMed
  41. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  42. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  43. Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed
  44. Kalim H, et al. 2020. Int J Rheum Dis. 23:620. PubMed
  45. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  46. Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed
  47. Mothe B, et al. 2020. Front Immunol. 1.029861111. PubMed
  48. Kothari H, et al. 2021. Sci Signal. 14:. PubMed
  49. Zelba H, et al. 2021. J Immunol. 206:580. PubMed
  50. Kim ML, et al. 2021. iScience. 24:103509. PubMed
RRID
AB_314073 (BioLegend Cat. No. 300505) AB_314074 (BioLegend Cat. No. 300506) AB_2562052 (BioLegend Cat. No. 300538)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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