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Brilliant Violet 421™ anti-human CD4 Antibod
产品名称：
Brilliant Violet 421™ anti-human CD4 Antibod
产品类别：
抗体
产品编号：
300531
产品应用：
300531
[价格]
规格 价格 库存
25tests ￥ 2660 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC?- Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L?per million cells in 100 ?L?staining volume or 5 ?L?per 100 ?L?of whole blood.?It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.? This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  11. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Afshar M, et al. 2015. Alcohol. 49:57. PubMed
  2. Nakano M, et al. 2021. Front Immunol. 12:713225. PubMed
  3. Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
  4. Massafra V, et al. 2021. J Immunol. 207:493. PubMed
  5. Pellett Madan R, et al. 2020. Clin Transplant. 34:e14021. PubMed
  6. Mimitou EP, et al. 2021. Nat Biotechnol. 39:1246. PubMed
  7. Kerstein A, et al. 2016. J Autoimmun. S0896-8411(16)30186-X. PubMed
  8. Juno JA, et al. 2020. Nat Med. 26:1428. PubMed
  9. Mederacke YS, et al. 2019. Sci Rep. 1.084027778. PubMed
  10. Balle C, et al. 2022. J Clin Invest. :. PubMed
  11. Vanuytsel K, et al. 2022. Nat Commun. 13:1103. PubMed
  12. Cornelius C et al. 2016. EBioMedicine. 11:58-67 . PubMed
  13. Pissani F, et al. 2018. J Virol. 92:e01143. PubMed
  14. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
  15. Tai YT, et al. 2019. Leukemia. 33:426. PubMed
  16. Chen Y, et al. 2021. Front Mol Biosci. 8:777370. PubMed
  17. Sam J, et al. 2020. Front Oncol. 10:575737. PubMed
RRID
AB_10900084 (BioLegend Cat. No. 300531) AB_10965645 (BioLegend Cat. No. 300532)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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