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APC/Cyanine7 anti-human CD4 Antibody
产品名称：
APC/Cyanine7 anti-human CD4 Antibody
产品类别：
抗体
产品编号：
300517
产品应用：
300517
[价格]
规格 价格 库存
25tests ￥ 1624 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.? This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  11. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed
  2. Bilich T, et al. 2021. Cancer Discov. 11:1982. PubMed
  3. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  4. Swadling L, et al. 2020. Cell Rep. 30:687. PubMed
  5. Yoshitomi H, et al. 2018. Nat Commun. 2.9875. PubMed
  6. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  7. Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
  8. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  9. Wefers C, et al. 2019. Front Immunol. 9:3156. PubMed
  10. Yarzabek B et al. 2018. eLife. 7 pii: e34961. PubMed
  11. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  12. Ogishi M, et al. 2021. Nat Med. 27:1646. PubMed
  13. Lübke M, et al. 2020. J Exp Med. 217:00:00. PubMed
  14. D, et al. 2016. Tuberculosis. 95: 470-475. PubMed
  15. Masuda H, et al. 2014. J Am Heart Assoc. 3:743. PubMed
  16. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  17. Geng J, et al. 2018. Elife. 7:e36341. PubMed
  18. Bauer J, et al. 2022. Nat Commun. 13:6401. PubMed
  19. Iannetta M, et al. 2016. PLoS One. 11: 0160277. PubMed
  20. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  21. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  22. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  23. Bilich T, et al. 2021. Science Translational Medicine. 13(590):. PubMed
  24. Goodwin M, et al. 2020. Sci Adv. 6:eaaz0571. PubMed
  25. Sayin I, et al. 2018. J Exp Med. 7:40286. PubMed
  26. Naidoo KK, et al. 2020. PLoS One. 15:e0242448. PubMed
  27. García-Espinoza JA, et al. 2022. Curr Issues Mol Biol. 44:764. PubMed
  28. Heitmann JS, et al. 2021. Nature. Online ahead of print. PubMed
  29. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  30. Kuo HH, et al. 2018. Immunity. 48:1183. PubMed
  31. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  32. Luo Y, et al. 2021. Front Immunol. 12:761209. PubMed
  33. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  34. Whitfield SJC, et al. 2017. J Immunol. 198:3989. PubMed
  35. Einkauf KB, et al. 2019. J Clin Invest. 129:988. PubMed
  36. Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed
  37. Jiang C, et al. 2020. Nature. 261:585. PubMed
  38. Einkauf KB, et al. 2022. Cell. 185:266. PubMed
  39. Brudno JN, et al. 2020. Nat Med. 270:26. PubMed
  40. Qi Y, et al. 2012. PLoS One. 7:e39072. PubMed
  41. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  42. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
RRID
AB_314085 (BioLegend Cat. No. 300517) AB_314086 (BioLegend Cat. No. 300518)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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