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APC anti-human CD4 Antibody
产品名称：
APC anti-human CD4 Antibody
产品类别：
抗体
产品编号：
300514
产品应用：
300514
[价格]
规格 价格 库存
100tests ￥ 1008 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.? This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  11. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Penkava F, et al. 2020. Nat Commun. 3.76875. PubMed
  2. Tang M, et al. 2019. Biomed Res Int. 2019:1050285. PubMed
  3. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  4. Nakano Y, et al. 2017. PLoS Pathog. 13:e1006348. PubMed
  5. Carpenter RS, et al. 2019. Sci Rep. 9:19105. PubMed
  6. Wang F, et al. 2018. Oncogenesis. 7:41. PubMed
  7. Eleftheriadis T, et al. 2021. Int J Mol Sci. 22:. PubMed
  8. Koelsch K, et al. 2013. PLoS One. 8:50068. PubMed
  9. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  10. Fletcher H, et al. 2016. Nat Commun. 7: 11290. PubMed
  11. Tang-Huau TL, et al. 2018. Nat Commun. 9:2570. PubMed
  12. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
  13. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  14. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  15. Gludish DW, et al. 2020. Sci Rep. 10:19900. PubMed
  16. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  17. Stuart T, et al. 2019. Cell. 177:1888. PubMed
  18. Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
  19. Kerstein A, et al. 2016. J Autoimmun. S0896-8411(16)30186-X. PubMed
  20. Vyborova A, et al. 2022. Front Immunol. 13:915366. PubMed
  21. Ng CY, et al. 2022. EMBO Rep. 23:e54271. PubMed
  22. Jancewicz I, et al. 2021. Cancers (Basel). 13:. PubMed
  23. Martín Monreal MT, et al. 2021. Front Immunol. 12:716250. PubMed
  24. Souriant S, et al. 2019. Cell Rep. 26:3586. PubMed
  25. Lu Y, et al. 2021. Gastroenterology. 161:575. PubMed
  26. Reichwald K, et al. 2014. PLoS One. 9:105627. PubMed
  27. Usero L, et al. 2016. Diabetes. 65: 2356 - 2366. PubMed
  28. Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed
  29. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  30. André M, et al. 2010. J Immunol. 185:2710. PubMed
  31. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  32. Park MJ, et al. 2019. J Immunol. 203:127. PubMed
  33. Zuccato C, et al. 2022. Ther Adv Hematol. 13:20406207221100648. PubMed
  34. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  35. Huber JE, et al. 2020. J Immunol. 204:1101. PubMed
  36. Lundy SK, et al. 2018. Front Med (Lausanne). 0.38125. PubMed
  37. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  38. Iwata A, et al. 2017. Nat Immunol. 18:563. PubMed
  39. Lotfi S, et al. 2020. Retrovirology. 17:20. PubMed
  40. Ng CY, et al. 2022. EMBO Rep. 23:e55871. PubMed
  41. Huang LJ, et al. 2021. Immunity. 54(8):1728-1744.e7. PubMed
  42. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  43. Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
  44. Cui J, et al. 2019. Neuro Oncol. 21:1436. PubMed
  45. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
  46. Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed
  47. Ma S, et al. 2022. Nat Commun. 13:4118. PubMed
  48. Li B, et al. 2019. Oncogenesis. 8:17. PubMed
  49. Chang XL, et al. 2021. Nat Commun. 12:3343. PubMed
  50. Pawlicki JM, et al. 2021. Cancer Res. 81:3241. PubMed
  51. Olety B, et al. 2021. Sci Adv. 7:eabj7398. PubMed
RRID
AB_314082 (BioLegend Cat. No. 300514) AB_2564153 (BioLegend Cat. No. 300552) AB_2562051 (BioLegend Cat. No. 300537)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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