- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
SB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.? This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300569 - 300574).
- Additional Product Notes
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
- Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
- Deng MC, et al. 1995. Circulation 91:1647. (IHC)
- Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Lan RY, et al. 2006. Hepatology 43:729.
- Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
- Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
- Hubbard N, et al. 2016. Blood. 127: 2513 - 2522. PubMed
- Taura M, et al. 2019. Proc Natl Acad Sci U S A. 116:2282. PubMed
- AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
- Elmentaite R, et al. 2021. Nature. 597:250. PubMed
- Holbrook AK, et al. 2019. Physiol Rep. 7:e14234. PubMed
- Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
- Kilgour MK, et al. 2022. Nat Protoc. 17:2668. PubMed
- Presicce P, et al. 2011. PLoS One. 6:e28118. PubMed
- Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
- Lerrer S, et al. 2021. iScience. 24:103020. PubMed
- Wild K, et al. 2021. Nat Commun. 12:6720. PubMed
- Vikkurthi R, et al. 2022. Nat Microbiol. 7:974. PubMed
- Newton HS, et al. 2021. Mol Ther Methods Clin Dev. 21:133. PubMed
- Usmani SM et al. 2019. Cell host & microbe. 25(1):73-86 . PubMed
- Platten M, et al. 2021. Nature. 592:463. PubMed
- Simpfendorfer K, et al. 2012. Hum Mol Genet. 21:3918. PubMed
- Oberhardt V, et al. 2021. Nature. 597:268. PubMed
- Muller YD, et al. 2021. Front Immunol. 12:639818. PubMed
- Aguilar-Pimentel A, et al. 2017. PLoS One. 12(6):e0178563. PubMed
- Carnevale G,et al. 2017. Sci Rep.. 10.1038/s41598-017-14838-3. PubMed
- Dupraz L, et al. 2021. Cell Reports. 36(1):109332. PubMed
- Yuan Z, et al. 2016. J Virol. 90: 7728 - 7739. PubMed
- Presicce P, et al. 2012. PLoS One. 7:e42802. PubMed
- Vanoni G, et al. 2021. eLife. 10:00. PubMed
- Toor SM, et al. 2018. Clin Exp Immunol. 191:189. PubMed
- RRID
- AB_493743 (BioLegend Cat. No. 300526)
