Alexa Fluor® 488 anti-human CD4 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100tests	￥ 3752	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
ICC?- Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L?per million cells in 100 ?L?staining volume or 5 ?L?per 100 ?L?of whole blood.?It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections^3,4,5, blocking of T cell activation^1,2, and spatial biology (IBEX)^10,11.? This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
Deng MC, et al. 1995. Circulation 91:1647. (IHC)
Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Lan RY, et al. 2006. Hepatology 43:729.
Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed
Emmons TR, et al. 2021. Cancer Immunol Res. 9:790. PubMed
Kip P, et al. 2021. Nutrients. 13:. PubMed
Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
Pothlichet J, et al. 2020. J Clin Invest. 130:2872. PubMed

RRID

AB_389311 (BioLegend Cat. No. 300519)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor: MHC class II molecules, HIV gp120, IL-16
Cell Type: Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

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