Purified anti-human CD2 Antibody
- 产品名称：
- Purified anti-human CD2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 300202
- 产品应用：
- 300202
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Pig
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and blocking of T cell activation2. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 300239 & 300240).
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Aversa G, et al. 1987. Transplant. Proc. 19:277. (Block)
- Zaretsky AG, et al. 2009. J. Exp Med. 206:991. (IHC) PubMed
- Perona-Wright G, et al. 2010. Nat. Immunol. 11:520. (FC) PubMed
- Thummler K, et al. 2010. J. Leukoc. Biol. 88:1041.
- Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Product Citations
-
- Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
- Thümmler K, et al. 2010. J Leukoc Biol. 88:1041. PubMed
- Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed
- Ghosh S, et al. 2020. Cell Reports. 30(10):3434-3447. PubMed
- Colleen S McGary et al. 2017. Immunity. 47(4):776-788 . PubMed
- Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
- Harper JL, et al. 2020. Nat Med. 519:26. PubMed
- Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed
- Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
- Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed
- Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
- Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
- RRID
- AB_314026 (BioLegend Cat. No. 300202)
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 50 kD
- Distribution
-
T cells, NK cells, thymocytes, and thymic B cell subsets
- Function
- T cell activation, adhesion
- Ligand/Receptor
- CD58 (LFA-3), CD48, CD59, CD15
- Cell Type
- B cells, NK cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Bell G, et al. 1995. J. Immunol. 155:2805.
2. Bierer B, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Moingeon P, et al. 1989. Immunol. Rev. 111:111.
- Gene ID
- 914 View all products for this Gene ID
- UniProt
- View information about CD2 on UniProt.org
