Biotin anti-human CD2 Antibody
- 产品名称：
- Biotin anti-human CD2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 300203
- 产品应用：
- 300203
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Pig
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and blocking of T cell activation2. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 300239 & 300240).
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Aversa G, et al. 1987. Transplant. Proc. 19:277. (Block)
- Zaretsky AG, et al. 2009. J. Exp Med. 206:991. (IHC) PubMed
- Perona-Wright G, et al. 2010. Nat. Immunol. 11:520. (FC) PubMed
- Thummler K, et al. 2010. J. Leukoc. Biol. 88:1041.
- Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Product Citations
-
- Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
- Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
- Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
- Goods BA, et al. 2021. JCI Insight. 6: . PubMed
- Sun J et al. 2018. Cell stem cell. 23(3):355-369 . PubMed
- Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
- Zaretsky A, et al. 2009. J Exp Med. 206:991. PubMed
- Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
- Askenase MH, et al. 2021. Sci Immunol. 6:. PubMed
- RRID
- AB_314027 (BioLegend Cat. No. 300203) AB_314028 (BioLegend Cat. No. 300204)
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 50 kD
- Distribution
-
T cells, NK cells, thymocytes, and thymic B cell subsets
- Function
- T cell activation, adhesion
- Ligand/Receptor
- CD58 (LFA-3), CD48, CD59, CD15
- Cell Type
- B cells, NK cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Bell G, et al. 1995. J. Immunol. 155:2805.
2. Bierer B, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Moingeon P, et al. 1989. Immunol. Rev. 111:111.
- Gene ID
- 914 View all products for this Gene ID
- UniProt
- View information about CD2 on UniProt.org
