Ultra-LEAF™ Purified anti-mouse CD180 (RP105-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3780	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

BALB/c mouse splenocytes

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, Activ, Apop - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1-3, B cell activation^1,4,5, protection of B cells from apoptosis induced by radiation or dexamethasone and induction of Fas-independent apoptosis^1,5. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 117709 & 117710).

Application References (PubMed link indicates BioLegend citation)

Miyake K, et al. 1994. J. Exp. Med. 180:1217. (FC IP Activ Apop)
Miyake K, et al. 1995. J. Immunol. 154:3333. (IP)
Miyake K, et al. 1998. J. Immunol. 161:1348. (IP)
Chan VWF, et al. 1998. J. Exp. Med. 188:93. (Activ)
Yamashita Y, et al. 1996. J. Exp. Med. 184:113. (Activ Apop)
Fujita K, et al. 2012. Immunol Lett. 144:1. PubMed.

Product Citations

Smith LK, et al. 2021. Elife. 10:. PubMed

RRID

AB_2832420 (BioLegend Cat. No. 117709) AB_2832421 (BioLegend Cat. No. 117710)

Antigen Details

Structure: Member of the toll-like receptor family, 105 kD
Distribution: B cells, monocytes/macrophages, and dendritic cells
Function: Signaling protein involved in innate immunity
Ligand/Receptor: LPS
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Miyake K, et al. 1994. J. Exp. Med. 180 :1217.
2. Miyake K, et al. 1995. J. Immunol.. 154:3333.
3. Miyake K, et al. 1998. J. Immunol.. 161:1348.
4. Chan VWF, et al. 1998. J. Exp. Med. 188 :93.
5. Yamashita Y, et al. 1996. J. Exp. Med. 184:113.
Gene ID: 17079 View all products for this Gene ID
UniProt: View information about CD180 on UniProt.org

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