PE anti-mouse CD180 (RP105) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
200µg	￥ 5376	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

BALB/c mouse splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1-3, B cell activation^1,4,5, protection of B cells from apoptosis induced by radiation or dexamethasone and induction of Fas-independent apoptosis^1,5. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 117709 & 117710).

Application References (PubMed link indicates BioLegend citation)

Miyake K, et al. 1994. J. Exp. Med. 180:1217. (FC IP Activ Apop)
Miyake K, et al. 1995. J. Immunol. 154:3333. (IP)
Miyake K, et al. 1998. J. Immunol. 161:1348. (IP)
Chan VWF, et al. 1998. J. Exp. Med. 188:93. (Activ)
Yamashita Y, et al. 1996. J. Exp. Med. 184:113. (Activ Apop)
Fujita K, et al. 2012. Immunol Lett. 144:1. PubMed.

Product Citations

van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
Fujita K, et al. 2012. Immunol Lett. 144:1. PubMed

RRID

AB_2072827 (BioLegend Cat. No. 117706)

Antigen Details

Structure: Member of the toll-like receptor family, 105 kD
Distribution: B cells, monocytes/macrophages, and dendritic cells
Function: Signaling protein involved in innate immunity
Ligand/Receptor: LPS
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Miyake K, et al. 1994. J. Exp. Med. 180 :1217.
2. Miyake K, et al. 1995. J. Immunol.. 154:3333.
3. Miyake K, et al. 1998. J. Immunol.. 161:1348.
4. Chan VWF, et al. 1998. J. Exp. Med. 188 :93.
5. Yamashita Y, et al. 1996. J. Exp. Med. 184:113.
Gene ID: 17079 View all products for this Gene ID
UniProt: View information about CD180 on UniProt.org

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