Ultra-LEAF™ Purified anti-mouse CD51 Antibod
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD51 Antibod
- 产品类别：
- 抗体
- 产品编号：
- 104109
- 产品应用：
- 104109
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- BALB/c mouse IL-2-activated killer (LAK) cells
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IP, Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, and blocking1-3 of CD51 adhesion and LAK cell cytotoxicity. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104109 & 104110).
- Application References
(PubMed link indicates BioLegend citation) -
- Takahashi K, et al. 1990. J. Immunol. 145:4371. (IP Block)
- Narumiya S, et al. 1994. Int. Immunol. 6:139. (Block)
- Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
- Delpino MV, et al. 2012. J. Leukoc Biol 91:285. PubMed.
- Product Citations
-
- Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed
- RRID
- AB_2819797 (BioLegend Cat. No. 104109) AB_2819798 (BioLegend Cat. No. 104110)
- Structure
- Integrin family, associates with integrins β1, β3, β5 or β6, 140 kD
- Distribution
-
Activated T cells, polymorphonuclear granulocytes, blastocysts, osteoclasts
- Function
- Adhesion
- Ligand/Receptor
- Fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31
- Cell Type
- Granulocytes, Mesenchymal Stem Cells, Osteoclasts, T cells
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Maxfield SR, et al. 1989. J. Exp. Med. 169:2173.
3. Piali L, et al. 1995. J. Cell Biol. 130:451.
- Gene ID
- 16410 View all products for this Gene ID
- UniProt
- View information about CD51 on UniProt.org
