Purified anti-mouse Tim-4 Antibody
- 产品名称：
- Purified anti-mouse Tim-4 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 130002
- 产品应用：
- 130002
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse TIM4-Ig fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Customer reported
FA, Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: in vivo induction of auto-antibody production1 and blockade of dendritic cell Tim-42.
- Application References
(PubMed link indicates BioLegend citation) -
- Nakayama M, et al. 2009. Blood. 113:3821. (FA)
- Yeung MY, et al. 2013. J. Immunol. 191:4447. (Block)
- Product Citations
-
- Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
- Khan T, et al. 2013. J Immunol. 190:1433. PubMed
- Dasgupta D, et al. 2020. Gastroenterology. 159:1487. PubMed
- O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
- Ipseiz N, et al. 2020. EMBO J. 39:e103454. PubMed
- RRID
- AB_1227802 (BioLegend Cat. No. 130002)
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain
- Distribution
-
Unlike the other Tims, Tim-4 is only expressed by antigen-presenting cells, but not by T cells.
- Function
- Tim-4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by binding phosphatidylserine.
- Interaction
- Phosphatiylserine is the ligand of Tim-4 and Tim-4 is a ligand of Tim-1.
- Ligand/Receptor
- Phosphatidylserine, TIM1
- Cell Type
- Antigen-presenting cells
- Biology Area
- Immunology
- Antigen References
-
1. Kuchroo VK, et al. 2008. Nat. Rev. Immunol. 8:577
2. Miyanishi M, et al. 2007. Nature 450:435
3. Rodriguez-Manzanet R, et al. 2008. J. Immunol. 180:4706
- Gene ID
- 276891 View all products for this Gene ID
- UniProt
- View information about Tim-4 on UniProt.org
