Alexa Fluor® 647 anti-mouse Tim-4 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-mouse Tim-4 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 130007
- 产品应用：
- 130007
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse TIM4-Ig fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: in vivo induction of auto-antibody production1 and blockade of dendritic cell Tim-42.
- Application References
(PubMed link indicates BioLegend citation) -
- Nakayama M, et al. 2009. Blood. 113:3821. (FA)
- Yeung MY, et al. 2013. J. Immunol. 191:4447. (Block)
- Product Citations
-
- Scott CL et al. 2018. Immunity. 49(2):312-325 . PubMed
- De Schepper S et al. 2018. Cell. 175(2):400-415 . PubMed
- Troutman TD, et al. 2021. STAR Protocols. 2(1):100363. PubMed
- Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
- Remmerie A, et al. 2020. Immunity. 53:641. PubMed
- Baratin M, et al. 2017. Immunity. 47:349. PubMed
- Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
- Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
- Yu YR, et al. 2020. STAR Protocols. 11(3):e0150606. PubMed
- RRID
- AB_2201842 (BioLegend Cat. No. 130007) AB_2271648 (BioLegend Cat. No. 130008)
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain
- Distribution
-
Unlike the other Tims, Tim-4 is only expressed by antigen-presenting cells, but not by T cells.
- Function
- Tim-4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by binding phosphatidylserine.
- Interaction
- Phosphatiylserine is the ligand of Tim-4 and Tim-4 is a ligand of Tim-1.
- Ligand/Receptor
- Phosphatidylserine, TIM1
- Cell Type
- Antigen-presenting cells
- Biology Area
- Immunology
- Antigen References
-
1. Kuchroo VK, et al. 2008. Nat. Rev. Immunol. 8:577
2. Miyanishi M, et al. 2007. Nature 450:435
3. Rodriguez-Manzanet R, et al. 2008. J. Immunol. 180:4706
- Gene ID
- 276891 View all products for this Gene ID
- UniProt
- View information about Tim-4 on UniProt.org
