Ultra-LEAF™ Purified anti-mouse CD366 (Tim-3
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD366 (Tim-3
- 产品类别：
- 抗体
- 产品编号：
- 119731
- 产品应用：
- 119731
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block, IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 119731-119736).
- Application References
(PubMed link indicates BioLegend citation) -
- Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
- Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)
- RRID
- AB_2810361 (BioLegend Cat. No. 119731) AB_2810362 (BioLegend Cat. No. 119732) AB_2810363 (BioLegend Cat. No. 119733) AB_2810364 (BioLegend Cat. No. 119734) AB_2810365 (BioLegend Cat. No. 119735) AB_2810366 (BioLegend Cat. No. 119736)
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
- Distribution
-
Expressed on activated Th1 lymphocytes and CD11b+ macrophages
- Function
- May play a role in the development of immune responses and the development of Th1-mediated responses
- Ligand/Receptor
- Putative ligand on resting CD4+ lymphocytes
- Cell Type
- Macrophages, Th1
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.
- Gene ID
- 171285 View all products for this Gene ID
- UniProt
- View information about CD366 on UniProt.org
