PE/Fire™ 810 anti-mouse CD366 (Tim-3) Antibo
- 产品名称：
- PE/Fire™ 810 anti-mouse CD366 (Tim-3) Antibo
- 产品类别：
- 抗体
- 产品编号：
- 119745
- 产品应用：
- 119745
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Fire? 810 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
?
* PE/Fire? 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire? 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page. - Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 119731-119736).
- Application References
(PubMed link indicates BioLegend citation) -
- Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
- Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)
- RRID
- AB_2922462 (BioLegend Cat. No. 119745)
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
- Distribution
-
Expressed on activated Th1 lymphocytes and CD11b+ macrophages
- Function
- May play a role in the development of immune responses and the development of Th1-mediated responses
- Ligand/Receptor
- Putative ligand on resting CD4+ lymphocytes
- Cell Type
- Macrophages, Th1
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.
- Gene ID
- 171285 View all products for this Gene ID
- UniProt
- View information about CD366 on UniProt.org
