PE/Fire™ 640 anti-mouse CD366 (Tim-3) Antibo-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2310	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Fire? 640 under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire? 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for relevant formats) include: in vitro¹ and in vivo² blocking of Tim-3, and immunohistochemical staining of frozen sections². The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 119731-119736).

Application References (PubMed link indicates BioLegend citation)

Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)

RRID

AB_2927886 (BioLegend Cat. No. 119749)

Antigen Details

Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
Distribution: Expressed on activated Th1 lymphocytes and CD11b⁺ macrophages
Function: May play a role in the development of immune responses and the development of Th1-mediated responses
Ligand/Receptor: Putative ligand on resting CD4⁺ lymphocytes
Cell Type: Macrophages, Th1
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.
Gene ID: 171285 View all products for this Gene ID
UniProt: View information about CD366 on UniProt.org

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