PE anti-mouse CD366 (Tim-3) Antibody
- 产品名称：
- PE anti-mouse CD366 (Tim-3) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 119703
- 产品应用：
- 119703
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 119731-119736).
- Application References
(PubMed link indicates BioLegend citation) -
- Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
- Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)
- Product Citations
-
- Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
- Khalsa JK, et al. 2020. Nat Commun. 3.175. PubMed
- Thomas Ciucci et al. 2019. Immunity. 50(1):91-105 . PubMed
- Magen A, et al. 2019. Cell Rep. 29:3019. PubMed
- Palacios LM, et al. 2022. Biology (Basel). 11:. PubMed
- de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
- ?lvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
- Gu M, et al. 2021. Nat Immunol. 22:193. PubMed
- DeLong JH, et al. 2019. Immunohorizons. 3:13. PubMed
- Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
- Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
- Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
- Chin AL, et al. 2021. Nat Commun. 12:5138. PubMed
- Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
- Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
- LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
- Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
- Sandu I, et al. 2020. Cell Reports. 32(8):108078. PubMed
- Benci JL et al. 2016. Cell. 167(6):1540-1554 . PubMed
- Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
- Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
- Nakazawa Y, et al. 2021. Elife. 10:. PubMed
- Schofield L, et al. 2017. BMC Med. 10.1186/s12916-017-0883-8. PubMed
- Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
- Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
- RRID
- AB_345377 (BioLegend Cat. No. 119703) AB_345378 (BioLegend Cat. No. 119704)
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
- Distribution
-
Expressed on activated Th1 lymphocytes and CD11b+ macrophages
- Function
- May play a role in the development of immune responses and the development of Th1-mediated responses
- Ligand/Receptor
- Putative ligand on resting CD4+ lymphocytes
- Cell Type
- Macrophages, Th1
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.
- Gene ID
- 171285 View all products for this Gene ID
- UniProt
- View information about CD366 on UniProt.org
