APC anti-mouse CD366 (Tim-3) Antibody
- 产品名称：
- APC anti-mouse CD366 (Tim-3) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 119705
- 产品应用：
- 119705
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 119731-119736).
- Application References
(PubMed link indicates BioLegend citation) -
- Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
- Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)
- Product Citations
-
- Aggen DH, et al. 2020. Clin Cancer Res. . PubMed
- Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
- Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed
- Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
- van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
- Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
- Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
- Watson MJ, et al. 2021. Nature. 591:645. PubMed
- Nair S, et al. 2021. JCI Insight. 6:. PubMed
- Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed
- Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
- Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
- Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
- Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
- RRID
- AB_2561655 (BioLegend Cat. No. 119705) AB_2561656 (BioLegend Cat. No. 119706)
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
- Distribution
-
Expressed on activated Th1 lymphocytes and CD11b+ macrophages
- Function
- May play a role in the development of immune responses and the development of Th1-mediated responses
- Ligand/Receptor
- Putative ligand on resting CD4+ lymphocytes
- Cell Type
- Macrophages, Th1
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.
- Gene ID
- 171285 View all products for this Gene ID
- UniProt
- View information about CD366 on UniProt.org
