PerCP/Cyanine5.5 anti-mouse CD279 (PD-1) Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD279 (PD-1) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 109119
- 产品应用：
- 109119
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse PD-1 transfected BHK cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
The RMP1-30 antibody does not block the binding of PD-1 to B7-H1 and B7-DC1.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Matsumoto K, et al. 2004. J. Immunol. 172:2530.
- Raimondi G, et al. 2006. J. Immunol. 176:2808. (FC) PubMed
- King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed
- Product Citations
-
- Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
- He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
- Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
- He X, et al. 2022. Cancer Immunol Res. 10:314. PubMed
- De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
- Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
- Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
- Emily A Thompson et al. 2019. Cell reports. 26(11):2859-2867 . PubMed
- Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
- RRID
- AB_2566640 (BioLegend Cat. No. 109119) AB_2566641 (BioLegend Cat. No. 109120)
- Structure
- Ig superfamily, 50-55 kD
- Distribution
-
Subset of double negative thymocytes, activated T and B cells
- Function
- Lymphocyte clonal selection, peripheral tolerance
- Ligand/Receptor
- PD-L1 (B7-H1), PD-L2
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.
- Gene ID
- 18566 View all products for this Gene ID
- UniProt
- View information about CD279 on UniProt.org
