PE/Dazzle™ 594 anti-mouse CD279 (PD-1) Antib
- 产品名称：
- PE/Dazzle™ 594 anti-mouse CD279 (PD-1) Antib
- 产品类别：
- 抗体
- 产品编号：
- 109115
- 产品应用：
- 109115
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse PD-1 transfected BHK cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The RMP1-30 antibody does not block the binding of PD-1 to B7-H1 and B7-DC1.
- Application References
(PubMed link indicates BioLegend citation) -
- Matsumoto K, et al. 2004. J. Immunol. 172:2530.
- Raimondi G, et al. 2006. J. Immunol. 176:2808. (FC) PubMed
- King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed
- Product Citations
-
- André P et al. 2018. Cell. 175(7):1731-1743 . PubMed
- Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
- Sheikh AA, et al. 2019. Cell Rep. 28:1758. PubMed
- Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
- Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
- RRID
- AB_2566547 (BioLegend Cat. No. 109115) AB_2566548 (BioLegend Cat. No. 109116)
- Structure
- Ig superfamily, 50-55 kD
- Distribution
-
Subset of double negative thymocytes, activated T and B cells
- Function
- Lymphocyte clonal selection, peripheral tolerance
- Ligand/Receptor
- PD-L1 (B7-H1), PD-L2
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.
- Gene ID
- 18566 View all products for this Gene ID
- UniProt
- View information about CD279 on UniProt.org
