Brilliant Violet 421™ anti-mouse CD279 (PD-1
- 产品名称：
- Brilliant Violet 421™ anti-mouse CD279 (PD-1
- 产品类别：
- 抗体
- 产品编号：
- 109121
- 产品应用：
- 109121
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse PD-1 transfected BHK cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Application Notes
The RMP1-30 antibody does not block the binding of PD-1 to B7-H1 and B7-DC1.
- Application References
(PubMed link indicates BioLegend citation) -
- Matsumoto K, et al. 2004. J. Immunol. 172:2530.
- Raimondi G, et al. 2006. J. Immunol. 176:2808. (FC) PubMed
- King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed
- Product Citations
-
- Pingili AK, et al. 2021. Cell Reports. 35(12):109285. PubMed
- Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
- Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
- Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
- Sheikh AA, et al. 2019. Cell Rep. 28:1758. PubMed
- Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
- Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
- RRID
- AB_2687080 (BioLegend Cat. No. 109121)
- Structure
- Ig superfamily, 50-55 kD
- Distribution
-
Subset of double negative thymocytes, activated T and B cells
- Function
- Lymphocyte clonal selection, peripheral tolerance
- Ligand/Receptor
- PD-L1 (B7-H1), PD-L2
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.
- Gene ID
- 18566 View all products for this Gene ID
- UniProt
- View information about CD279 on UniProt.org
