Ultra-LEAF™ Purified anti-mouse CD279 (PD-1)-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1540	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse PD-1 transfected BHK cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC, WB, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections¹, Western blotting^2,6, inhibition of macrophages (but not B cells and dendritic cells) costimulated T cell proliferation^3,5, and blocking the binding of PD-1 to B7-H1 or B7-DC^3,4,8. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 114108). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 114110) with a lower endotoxin limit than standard LEAF? purified antibodies.

Application References (PubMed link indicates BioLegend citation)

Kanai, T., et al. 2003. J. Immunol. 171:4156. (FC, IHC)
Personal Communication (WB)
Yamazaki, T., et al. 2005. J. Immunol. 175:1586. (Costim)
Matsumoto, K., et al. 2004. J. Immunol. 172:2530. (Block)
Terrazas, L. I., et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
Meng, Q., et al. 2006. Invest Opthalmol Vis Sci. 47:444. (FC, WB) PubMed
Tsang JY, et al. 2011. Int Immunopharmacol. 11:604. PubMed
Takamura S, et al. 2010. J. Immunol. 184:4696. PubMed (Block)

Product Citations

Godoy–Calderón MJ, et al. 2019. Oncotarget. 4.9625. PubMed
Hardcastle J, et al. 2016. Neuro Oncology. 10.1093/neuonc/now179. PubMed
Dheilly E, et al. 2020. Cancer Cell. 37(5):674-689. PubMed

RRID

AB_2783054 (BioLegend Cat. No. 114109) AB_2564183 (BioLegend Cat. No. 114110) AB_2800575 (BioLegend Cat. No. 114119) AB_2800576 (BioLegend Cat. No. 114120) AB_2800577 (BioLegend Cat. No. 114121) AB_2800578 (BioLegend Cat. No. 114122)

Antigen Details

Structure: Ig superfamily, 50-55 kD
Distribution: Subset of double negative thymocytes, activated T and B cells
Function: Lymphocyte clonal selection, peripheral tolerance
Ligand/Receptor: PD-L1 (B7-H1), PD-L2
Cell Type: B cells, T cells, Thymocytes, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook, Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.
Gene ID: 18566 View all products for this Gene ID
UniProt: View information about CD279 on UniProt.org

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