PE anti-mouse CD279 (PD-1) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1876	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse PD-1 transfected BHK cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections¹, Western blotting^2,6, inhibition of macrophages (but not B cells and dendritic cells) costimulated T cell proliferation^3,5, and blocking the binding of PD-1 to B7-H1 or B7-DC^3,4,8. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 114108). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 114110) with a lower endotoxin limit than standard LEAF? purified antibodies.

Application References (PubMed link indicates BioLegend citation)

Kanai, T., et al. 2003. J. Immunol. 171:4156. (FC, IHC)
Personal Communication (WB)
Yamazaki, T., et al. 2005. J. Immunol. 175:1586. (Costim)
Matsumoto, K., et al. 2004. J. Immunol. 172:2530. (Block)
Terrazas, L. I., et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
Meng, Q., et al. 2006. Invest Opthalmol Vis Sci. 47:444. (FC, WB) PubMed
Tsang JY, et al. 2011. Int Immunopharmacol. 11:604. PubMed
Takamura S, et al. 2010. J. Immunol. 184:4696. PubMed (Block)

Product Citations

Park S, et al. 2021. Front Immunol. 11:620166. PubMed
Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
Vijay R, et al. 2021. J Exp Med. 218:. PubMed
Wagner AK, et al. 2022. iScience. 25:105137. PubMed
Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed

RRID

AB_2566725 (BioLegend Cat. No. 114117) AB_2566726 (BioLegend Cat. No. 114118)

Antigen Details

Structure: Ig superfamily, 50-55 kD
Distribution: Subset of double negative thymocytes, activated T and B cells
Function: Lymphocyte clonal selection, peripheral tolerance
Ligand/Receptor: PD-L1 (B7-H1), PD-L2
Cell Type: Thymocytes, T cells, B cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Cancer Biomarkers, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook, Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.
Gene ID: 18566 View all products for this Gene ID
UniProt: View information about CD279 on UniProt.org

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