Ultra-LEAF™ Purified anti-mouse/rat CD62P (P-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1778	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Thrombin activated rat platelets

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
ICC, IHC, WB, IP, ELISA, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L?volume or 100 ?L?of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence⁶, immunohistochemistry⁵, ELISA⁷, Western blotting⁴, immunoprecipitation⁴, and blocking in rats^1,2 and mice³.

Application References (PubMed link indicates BioLegend citation)

Walter UM, et al. 1997. Immunology. 92:290. (Block-rats)
Johnston B, et al. 1997. J. Immunol. 159:4514. (Block-rats)
Hickey MJ, et al. 1998. Circ. Res. 83:1124. (Block-mice)
Walter UM, et al. 1997. Hybridoma. 16:249. (WB, IP)
Alvarez A, et al. 2004. Blood 104:402. (IHC)
Piccio L, et al. 2002. J. Immunol. 168:1940. (IF)
Walter UM, et al. 1997. Hybridoma. 16:249. (ELISA)

Product Citations

Perdomo J, et al. 2019. Nat Commun. 10:1322. PubMed

RRID

AB_2565816 (BioLegend Cat. No. 148307) AB_2565817 (BioLegend Cat. No. 148308)

Antigen Details

Structure: Type I transmembrane glycoprotein, 140 kD
Distribution: Activated platelets, megakaryocytes, endothelial cells
Function: Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
Ligand/Receptor: CD162 (PSGL-1), CD24 and sialylated Lewis X
Cell Type: Endothelial cells, Megakaryocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. Proc. Natl. Acad. Sci. USA 91:7390.
Gene ID: 20344 View all products for this Gene ID 25651 View all products for this Gene ID
UniProt: View information about CD62P on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们