APC anti-mouse/rat CD62P (P-selectin) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2254	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Thrombin activated rat platelets

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence⁶, immunohistochemistry⁵, ELISA⁷, Western blotting⁴, immunoprecipitation⁴, and blocking in rats^1,2 and mice³.

Application References (PubMed link indicates BioLegend citation)

Walter UM, et al. 1997. Immunology. 92:290. (Block-rats)
Johnston B, et al. 1997. J. Immunol. 159:4514. (Block-rats)
Hickey MJ, et al. 1998. Circ. Res. 83:1124. (Block-mice)
Walter UM, et al. 1997. Hybridoma. 16:249. (WB, IP)
Alvarez A, et al. 2004. Blood 104:402. (IHC)
Piccio L, et al. 2002. J. Immunol. 168:1940. (IF)
Walter UM, et al. 1997. Hybridoma. 16:249. (ELISA)

Product Citations

Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed
Malladi N, et al. 2022. J Diabetes Res. 2021:6404438. PubMed
Nicolai L, et al. 2021. Haematologica. Online ahead of print. PubMed
Cornelius DC, et al. 2020. PLoS One. 15:e0234039. PubMed
Gao Y, et al. 2020. Sci Adv. 6:eaba0588. PubMed

RRID

AB_2565272 (BioLegend Cat. No. 148303) AB_2565273 (BioLegend Cat. No. 148304)

Antigen Details

Structure: Type I transmembrane glycoprotein, 140 kD
Distribution: Activated platelets, megakaryocytes, endothelial cells
Function: Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
Ligand/Receptor: CD162 (PSGL-1), CD24 and sialylated Lewis X
Cell Type: Endothelial cells, Megakaryocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. Proc. Natl. Acad. Sci. USA 91:7390.
Gene ID: 20344 View all products for this Gene ID 25651 View all products for this Gene ID
UniProt: View information about CD62P on UniProt.org

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