Ultra-LEAF™ Purified anti-mouse/rat CD62E (E-上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 4858	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Rat E-selectin transfected chinese hamster ovary cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

IHC-F - Quality tested
ICC, WB, IP, ELISA, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^4,5, Western blotting¹, immunoprecipitation¹, ELISA⁷, and blocking^1,2,3. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 148803).

Application References (PubMed link indicates BioLegend citation)

Walter UM, et al. 1997. Hybridoma 16:249. (Block, WB, IP)
Walter UM, et al. 1997. Immunology 92:290. (Block, Rat Reactivity)
Hickey MJ, et al. 1998. Circ. Res. 83:1124. (Block, Mouse Reactivity)
Bernardes-Silva M, et al. 2001. J. Cereb. Blood Flow Metab. 21:1115. (IHC)
Alvarez A, et al. 2004. Blood 104:402. (IHC, Rat Reactivity)
Piccio L, et al. 2002. J. Immunol. 168:1940. (IF)
Walter UM, et al. 1997. in Hybridoma 16:355. (ELISA)

RRID

AB_2565818 (BioLegend Cat. No. 148803)

Antigen Details

Structure

Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain

Distribution

Highly expressed on activated endothelial cells

Function

Involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis

Ligand/Receptor

Sialyl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported to interact with CD62E

Cell Type

Endothelial cells

Biology Area

Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

Collins T, et al. 1991. J. Biol. Chem. 266:2466
Bevilacqua MP, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9238
Berg EL, et al. 1991. J. Exp. Med. 174:1461.
Lawrence MB, et al. 1993. J. Immunol. 151:6338.
Walz G, et al. 1990. Science 250:1132

Gene ID

20351 View all products for this Gene ID

UniProt

View information about CD62E on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们