Purified anti-mouse CD4 (Maxpar® Ready) Antibo-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1484	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

BALB/c mouse thymocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4⁺ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4⁺ cells¹, and immunohistochemistry of acetone-fixed frozen tissue sections^2,3,11 and paraffin-embedded sections¹¹. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
León-Ponte M, et al. 2007. Blood 109:3139. (FC)
Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
Shigeta A, et al. 2008. Blood 112:4915. PubMed
Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)

Product Citations

McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed
Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed

RRID

AB_2562762 (BioLegend Cat. No. 100561)

Antigen Details

Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, T cell subset
Function: TCR co-receptor, T cell activation
Ligand/Receptor: MHC class II molecule
Cell Type: Dendritic cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
Gene ID: 12504 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们