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PerCP anti-mouse CD4 Antibody
产品名称：
PerCP anti-mouse CD4 Antibody
产品类别：
抗体
产品编号：
100537
产品应用：
100537
[价格]
规格 价格 库存
25µg ￥ 1386 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  2. Yang R, et al. 2020. Cell Death Dis. 0.877083333. PubMed
  3. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  4. Martens R, et al. 2020. Nat Commun. 11:1114. PubMed
  5. Swadling L, et al. 2020. Cell Rep. 30:687. PubMed
  6. Fitzgerald B, et al. 2021. Cell Rep Methods. 1:. PubMed
  7. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  8. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  9. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  10. Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed
  11. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  12. Roy S, et al. 2020. Sci Rep. 10:10992. PubMed
  13. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  14. Shrivastava T, et al. 2021. Front Immunol. 12:641447. PubMed
  15. Thauland T, et al. 2014. J Immunol. 193:5894. PubMed
  16. K?chl R, et al. 2020. Elife. 9:00. PubMed
  17. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  18. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  19. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  20. Jacque E, et al. 2015. J Exp Med. 212:883. PubMed
  21. Schuldt N, et al. 2015. PLoS One. 10: 0145762. PubMed
  22. Sood S, et al. 2016. J Immunol. 197: 429 - 440. PubMed
  23. Van Gool F, et al. 2019. Immunity. 50:362. PubMed
  24. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  25. Robles-Oteiza C, et al. 2021. Dis Model Mech. 14:. PubMed
  26. Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
  27. Riva A, et al. 2017. PLoS One.. 10.1371/journal.pone.0181964. PubMed
  28. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  29. Hartwig S, et al. 2014. PLoS One. 9:90720. PubMed
  30. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  31. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  32. Menon M, et al. 2014. PLoS Genet. 10:1004558. PubMed
  33. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  34. Jacque E, et al. 2014. J Exp Med. 211:2085. PubMed
  35. Tao Z, et al. 2022. Cells. 11:. PubMed
  36. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  37. Tang L, et al. 2022. Front Immunol. 12:770402. PubMed
  38. Sturmlechner I, et al. 2021. Science. 374:eabb3420. PubMed
  39. Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
  40. Yuan M, et al. 2017. Hum Reprod. 32:94. PubMed
  41. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
RRID
AB_893331 (BioLegend Cat. No. 100537) AB_893325 (BioLegend Cat. No. 100538)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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