PE/Cyanine5 anti-mouse CD4 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1148	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

BALB/c mouse thymocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4⁺ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4⁺ cells¹, and immunohistochemistry of acetone-fixed frozen tissue sections^2,3,11 and paraffin-embedded sections¹¹. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
León-Ponte M, et al. 2007. Blood 109:3139. (FC)
Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
Shigeta A, et al. 2008. Blood 112:4915. PubMed
Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)

Product Citations

Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
Li F, et al. 2022. Mediators Inflamm. 2022:5782922. PubMed
Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
Yankova E, et al. 2021. Nature. 593:597. PubMed
Baranwal G, et al. 2021. Physiol Rep. 9:e15094. PubMed
Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed
Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed

RRID

AB_312716 (BioLegend Cat. No. 100513) AB_312717 (BioLegend Cat. No. 100514)

Antigen Details

Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, T cell subset
Function: TCR co-receptor, T cell activation
Ligand/Receptor: MHC class II molecule
Cell Type: Dendritic cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
Gene ID: 12504 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

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