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Brilliant Violet 421™ anti-mouse CD4 Antibod
产品名称：
Brilliant Violet 421™ anti-mouse CD4 Antibod
产品类别：
抗体
产品编号：
100543
产品应用：
100543
[价格]
规格 价格 库存
125µL ￥ 2394 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the ?g size, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L?volume. For immunocytochemistry staining using ?l sizes, the suggested use of this reagent is 5 ?L?per million cells in 100 ?L?staining volume or 5 ?L?per 100 ?L?of whole blood.?For immunohistochemistry on frozen tissue sections using the ?g size, a concentration range of 5.0 - 10 ?g/mL?is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  2. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  3. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  4. Lechuga-Vieco AV, et al. 2020. Sci Adv. 6:eaba5345. PubMed
  5. Prasad M, et al. 2020. Cell Mol Immunol. . PubMed
  6. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  7. Wang D, et al. 2018. Immunity. 48:659. PubMed
  8. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  9. Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed
  10. Park S, et al. 2021. Front Immunol. 11:620166. PubMed
  11. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  12. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  13. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  14. Stein RR, et al. 2018. Elife. 7:e30916. PubMed
  15. Rodriguez-Ruiz M, et al. 2016. Cancer Res . 76: 5994 - 6005. PubMed
  16. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  17. K?chl R, et al. 2020. Elife. 9:00. PubMed
  18. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  19. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  20. Parayath NN, et al. 2020. Nat Commun. 4.680555556. PubMed
  21. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  22. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  23. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  24. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
  25. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  26. Chen EW, et al. 2018. Sci Rep. 8:10046. PubMed
  27. Kim D, et al. 2020. Immunity. 53(3):581-596.e5. PubMed
  28. Lopes N, et al. 2022. Elife. 11:. PubMed
  29. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
  30. Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
  31. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  32. Wang Y, et al. 2020. Immunity. 53(6):1168-1181.e7. PubMed
  33. Stone S et al. 2018. Glia. 66(7):1331-1345 . PubMed
  34. Chen EW, et al. 2019. Front Immunol. 10:1718. PubMed
  35. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  36. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
RRID
AB_10898318 (BioLegend Cat. No. 100543) AB_2563052 (BioLegend Cat. No. 100563) AB_11219790 (BioLegend Cat. No. 100544)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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