热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
APC anti-mouse CD4 Antibody
产品名称：
APC anti-mouse CD4 Antibody
产品类别：
抗体
产品编号：
100515
产品应用：
100515
[价格]
规格 价格 库存
25µg ￥ 462 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  2. Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed
  3. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  4. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  5. Hirako I, et al. 2016. Nat Commun. 7:13277. PubMed
  6. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  7. Suzuki K, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:119. PubMed
  8. Lee S, et al. 2021. JCI Insight. 6:. PubMed
  9. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  10. Fisher E, et al. 2021. Front Immunol. 11:602254. PubMed
  11. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  12. Lee J, et al. 2020. Gut Microbes. 1:. PubMed
  13. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  14. Matsumoto M, et al. 2007. J Immunol . 178:2499. PubMed
  15. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  16. Rodriguez-Manzanet R, et al. 2010. Proc Natl Acad Sci U S A. 107:8706. PubMed
  17. Zhao B, et al. 2020. Nat Commun. 11:908. PubMed
  18. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  19. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  20. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  21. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  22. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  23. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  24. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  25. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  26. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  27. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  28. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  29. Li Y, et al. 2020. Nat Commun. 11:2781. PubMed
  30. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  31. Shigeta A, et al. 2008. Blood. 112:4915. PubMed
  32. van der Lelie D, et al. 2021. Nat Commun. 12:3105. PubMed
  33. Kang S, et al. 2021. Bio Protoc. 11:e4029. PubMed
  34. Nakajima A, et al. 2014. PLoS One. 9:105904. PubMed
  35. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  36. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  37. Park S, et al. 2013. J Immunol. 190:3480. PubMed
  38. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  39. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  40. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  41. Anger-Góra N, et al. 2021. Oncol Lett. 22:582. PubMed
  42. Koikawa K, et al. 2021. Cell. 184(18):4753-4771.e27. PubMed
  43. Rovira-Clavé X, et al. 2016. J Leukoc Biol. 99: 143 - 152. PubMed
  44. Tsumiyama K, et al. 2013. J Immunol. 191:91. PubMed
  45. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  46. Lin YW, et al. 2022. Mol Med Rep. 26:. PubMed
  47. Moutuou MM, et al. 2020. Methods Mol Biol. 2111:267. PubMed
  48. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  49. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  50. Biton J, et al. 2016. J Immunol. 197: 1708 - 1719. PubMed
  51. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  52. Wu S, et al. 2021. Nat Cancer. 2:189. PubMed
  53. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  54. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  55. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  56. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  57. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  58. Xiao u, et al. 2015. J Immunol. 195: 4218 - 4227. PubMed
  59. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  60. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  61. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  62. Zaborsky N, et al. 2010. J Immunol. 184:725. PubMed
  63. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  64. Liu J, et al. 2021. Front Immunol. 12:733808. PubMed
  65. Ene CI, et al. 2020. Neuro Oncol. 639:22. PubMed
  66. Lee Y, et al. 2012. Nat Immunol. 13:991. PubMed
  67. Ulaganathan VK, et al. 2020. Sci Rep. 10:8453. PubMed
  68. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  69. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  70. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  71. Kramer K, et al. 2017. Mol Ther. 25(1):62-70. PubMed
  72. Takahashi T, et al. 2017. J Exp Med. 10.1084/jem.20160247. PubMed
  73. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  74. Denzin LK et al. 2017. Immunity. 47(2):310-322 . PubMed
  75. Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
  76. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  77. Guo X, et al. 2020. J Cell Mol Med. . PubMed
  78. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  79. Berry G, Waldner H 2013. J Vis Exp. 75: 50389. PubMed
  80. St Clair JR, et al. 2018. Nat Commun. 9:1742. PubMed
  81. Sudo K, et al. 2021. Stem Cell Res. 56:102531. PubMed
  82. Skirecki T, et al. 2020. JCI Insight. 5:. PubMed
  83. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  84. Zaiss M, et al. 2015. Immunity. 43: 998-1010. PubMed
  85. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  86. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  87. García–Rodríguez S, et al. 2018. Sci Rep. 2.664583333. PubMed
  88. Wen X, et al. 2021. World J Gastroenterol. 27:2834. PubMed
  89. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  90. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  91. Palaniyandi S, et al. 2013. PLoS One. 8:61841. PubMed
  92. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
RRID
AB_312718 (BioLegend Cat. No. 100515) AB_312719 (BioLegend Cat. No. 100516)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线