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首页 > 抗体蛋白 > 抗体
Alexa Fluor® 700 anti-mouse CD4 Antibody
产品名称：
Alexa Fluor® 700 anti-mouse CD4 Antibody
产品类别：
抗体
产品编号：
100536
产品应用：
100536
[价格]
规格 价格 库存
100µg ￥ 3010 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  2. Wu SY, et al. 2022. Mol Cancer. 21:84. PubMed
  3. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  4. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  5. Singh S, et al. 2019. PLoS Pathog. 15:e1007460. PubMed
  6. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  7. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  8. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  9. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  10. Qiu F, et al. 2022. J Cancer. 13:2893. PubMed
  11. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  12. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  13. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
  14. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  15. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  16. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  17. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  18. Reed J, et al. 2020. Bio Protoc. 10:e3607. PubMed
  19. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  20. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  21. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
  22. Hanna CC, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  23. Jain RW, et al. 2018. Cell Rep. 25:3342. PubMed
  24. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  25. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  26. Altin J, et al. 2011. J Allergy Clin Immunol. 127:1277. PubMed
  27. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  28. Siegemund S, et al. 2015. PLoS One. 10:124661. PubMed
  29. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  30. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  31. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
  32. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  33. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  34. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  35. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
  36. Schulze B, et al. 2016. Pathog Dis. 74: ftw020. PubMed
  37. Hendrikx S et al. 2019. Cell reports. 26(5):1227-1241 . PubMed
  38. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  39. Mykk?nen O, et al. 2014. PLoS One. 9:114790. PubMed
  40. Regli IB, et al. 2020. Cell Reports. 31(10):107746. PubMed
  41. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  42. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  43. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  44. Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
  45. Song W, et al. 2022. Immunity. 55:290. PubMed
RRID
AB_493701 (BioLegend Cat. No. 100536)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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