PerCP/Cyanine5.5 anti-mouse CD4 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD4 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 116011
- 产品应用：
- 116011
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- BALB/c mouse thymocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
RM4-4 antibody does not block the binding of GK1.5 and RM4-5 antibodies to CD4 T cells. For immunohistochemistry applications, the RM4-5 (Cat. No. 100506) and GK1.5 (Cat. No. 100402) antibodies are recommended.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Bendelac A. 1995. Curr. Opin. Immunol. 7:367.
- Norian LA and Allen PM. 2004. J. Immunol. 173:835.
- Product Citations
-
- Zhou R, et al. 2022. EBioMedicine. 75:103762. PubMed
- Tian J, et al. 2021. Autophagy. 17:2586. PubMed
- Gilleron J et al. 2018. Cell reports. 25(12):3329-3341 . PubMed
- Stathopoulou C, et al. 2020. Immunity. 49(2):247-263.e7.. PubMed
- Sugimoto C, et al. 2022. Elife. 11:. PubMed
- Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
- Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
- Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
- Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
- Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
- Yu X, et al. 2019. Nat Commun. 10:574. PubMed
- Kyburz A, et al. 2017. Clin Exp Allergy. 47:1331. PubMed
- Gupta S, et al. 2022. Life Sci. 288:120182. PubMed
- Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
- RRID
- AB_2563022 (BioLegend Cat. No. 116011) AB_2563023 (BioLegend Cat. No. 116012)
- Structure
- Ig superfamily, 55 kD
- Distribution
-
Majority of thymocytes, T cell subset
- Function
- TCR co-receptor, T cell activation
- Ligand/Receptor
- MHC class II molecule
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A,?et al.?1997. The Leukocyte Antigen FactsBook Academic Press.?
2. Bierer BE,?et al.?1989.?Annu. Rev. Immunol.?7:579.?
3. Janeway CA. 1992.?Annu. Rev. Immunol.?10:645.
- Gene ID
- 12504 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org
