PE/Cyanine7 anti-mouse CD4 Antibody
- 产品名称：
- PE/Cyanine7 anti-mouse CD4 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 116015
- 产品应用：
- 116015
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- BALB/c mouse thymocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
RM4-4 antibody does not block the binding of GK1.5 and RM4-5 antibodies to CD4 T cells. For immunohistochemistry applications, the RM4-5 (Cat. No. 100506) and GK1.5 (Cat. No. 100402) antibodies are recommended.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Bendelac A. 1995. Curr. Opin. Immunol. 7:367.
- Norian LA and Allen PM. 2004. J. Immunol. 173:835.
- Product Citations
-
- Pani F, et al. 2021. Endocrinology. 162:. PubMed
- Page N, et al. 2018. Immunity. 48:937. PubMed
- Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
- Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
- He L, et al. 2019. iScience. 0.790972222. PubMed
- Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
- Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
- Yang C, et al. 2020. Cell Host Microbe. 467:27. PubMed
- Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
- Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
- RRID
- AB_2563110 (BioLegend Cat. No. 116015) AB_2563111 (BioLegend Cat. No. 116016)
- Structure
- Ig superfamily, 55 kD
- Distribution
-
Majority of thymocytes, T cell subset
- Function
- TCR co-receptor, T cell activation
- Ligand/Receptor
- MHC class II molecule
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A,?et al.?1997. The Leukocyte Antigen FactsBook Academic Press.?
2. Bierer BE,?et al.?1989.?Annu. Rev. Immunol.?7:579.?
3. Janeway CA. 1992.?Annu. Rev. Immunol.?10:645.
- Gene ID
- 12504 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org
