Pacific Blue™ anti-mouse CD4 Antibody
- 产品名称：
- Pacific Blue™ anti-mouse CD4 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 116007
- 产品应用：
- 116007
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- BALB/c mouse thymocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
RM4-4 antibody does not block the binding of GK1.5 and RM4-5 antibodies to CD4 T cells. For immunohistochemistry applications, the RM4-5 (Cat. No. 100506) and GK1.5 (Cat. No. 100402) antibodies are recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Bendelac A. 1995. Curr. Opin. Immunol. 7:367.
- Norian LA and Allen PM. 2004. J. Immunol. 173:835.
- Product Citations
-
- Shapiro MR, et al. 2020. Front Immunol. 1.972222222. PubMed
- Gubser C, et al. 2016. Sci Rep. 6: 25758. PubMed
- Soto-Díaz K, et al. 2021. Front Immunol. 12:734349. PubMed
- Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
- Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
- Wang H, et al. 2020. Nat Mater. 1.655555556. PubMed
- Prizant H, et al. 2021. Cell Reports. 36(6):109523. PubMed
- Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
- Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
- Lytle NK et al. 2019. Cell. 177(3):572-586 . PubMed
- Richardson M, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
- Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
- RRID
- AB_11147758 (BioLegend Cat. No. 116007) AB_11149680 (BioLegend Cat. No. 116008)
- Structure
- Ig superfamily, 55 kD
- Distribution
-
Majority of thymocytes, T cell subset
- Function
- TCR co-receptor, T cell activation
- Ligand/Receptor
- MHC class II molecule
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A,?et al.?1997. The Leukocyte Antigen FactsBook Academic Press.?
2. Bierer BE,?et al.?1989.?Annu. Rev. Immunol.?7:579.?
3. Janeway CA. 1992.?Annu. Rev. Immunol.?10:645.
- Gene ID
- 12504 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org
