Ultra-LEAF? Purified anti-mouse CD2 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2856	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

BALB/c mouse thymocytes

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, Block, Costim - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation¹, blocking of T cell A.I.C.D.², immunoprecipitation³, and co-induction of thymocyte maturation⁴. The RM2-5 antibody can block CD2-mediated cell-cell adhesion. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 100118 and 100119).

Application References (PubMed link indicates BioLegend citation)

Nakamura T, et al. 1990. J. Immunol. 145:3628. (Block)
Ayroldi E, et al. 1997. Blood 89:3717. (Block)
Criado G, et al. 1996. Eur. J. Immunol. 26:1228. (IP)
Cibotti R, et al. 1997. Immunity 6:245. (Costim)

RRID

AB_2832256 (BioLegend Cat. No. 100118) AB_2832257 (BioLegend Cat. No. 100119)

Antigen Details

Structure: Ig superfamily, 45-58 kD
Distribution: T cells, thymocytes, NK cells, B cells
Function: T cell activation, adhesion
Ligand/Receptor: CD48, lck
Cell Type: B cells, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis SJ, et al. 1996. Immunol. Today 17:177.
3. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
Gene ID: 12481 View all products for this Gene ID
UniProt: View information about CD2 on UniProt.org

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