PE/Cyanine7 anti-mouse CD2 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1484	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

BALB/c mouse thymocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation¹, blocking of T cell A.I.C.D.², immunoprecipitation³, and co-induction of thymocyte maturation⁴. The RM2-5 antibody can block CD2-mediated cell-cell adhesion. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 100118 and 100119).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Nakamura T, et al. 1990. J. Immunol. 145:3628. (Block)
Ayroldi E, et al. 1997. Blood 89:3717. (Block)
Criado G, et al. 1996. Eur. J. Immunol. 26:1228. (IP)
Cibotti R, et al. 1997. Immunity 6:245. (Costim)

Product Citations

Kobayashi T, et al. 2019. Cell. 176:982. PubMed
Patenaude J, Perreault C 2016. J Immunol. 196: 4760 - 4770. PubMed
Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
Ulu?kan ?, et al. 2020. Cell Reports. 29(4):844-859.e3.. PubMed

RRID

AB_2563091 (BioLegend Cat. No. 100113) AB_2563092 (BioLegend Cat. No. 100114)

Antigen Details

Structure: Ig superfamily, 45-58 kD
Distribution: T cells, thymocytes, NK cells, B cells
Function: T cell activation, adhesion
Ligand/Receptor: CD48, lck
Cell Type: B cells, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis SJ, et al. 1996. Immunol. Today 17:177.
3. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
Gene ID: 12481 View all products for this Gene ID
UniProt: View information about CD2 on UniProt.org

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