PE anti-mouse CD153 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1372	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

CHO cells transfected with mouse CD153 gene (Tnfsf8)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen sections, and in vivo blocking of CD30 binding to CD30 ligand (CD153)^1-3,5. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 106408).

Application References (PubMed link indicates BioLegend citation)

Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519.
Seko Y, et al. 2001. J. Pathol. 195:593.
Akiba H, et al. 2000. J. Exp. Med. 191:375.
Zhang H and GG Meadows 2005. J. Leukocyte Biol. 78:1070.PubMed
Zeiser R, et al. 2007. Blood 109:2225.

Product Citations

Kurosawa M, et al. 2021. Int J Mol Sci. 22:. PubMed
Giannattasio G, et al. 2010. J Immunol. 185:4430. PubMed
Meadows H 2005. J Leukoc Biol. 78:1070. PubMed

RRID

AB_2207502 (BioLegend Cat. No. 106405)

Antigen Details

Structure: TNF superfamily, 39-40 kD
Distribution: Activated T cells
Function: T-B cell costimulation
Ligand/Receptor: CD30
Cell Type: T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Horie R, et al. 1998. Semin. Immunol. 106:457.
3. Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519.
4. Shanebeck KD, et al. 1995. Eur. J. Immunol. 25:2147.
5. Bowen MA, et al. 1996. J. Immunol. 156:442.
Gene ID: 21949 View all products for this Gene ID
UniProt: View information about CD153 on UniProt.org

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