Ultra-LEAF™ Purified anti-mouse CD252 (OX40 -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1540	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Rat NRK-52E cell line transfected with mouse OX-40L

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC -?Quality tested
IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The RM134L antibody can block the costimulatory activity of OX40L. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections³, and in vivo and in vitro blocking of OX-40L-OX-40 functional interaction^1,2,4. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 108808).

Application References (PubMed link indicates BioLegend citation)

Akiba H, et al. 1999. J. Immunol. 162:7058. (Block)
Akiba H, et al. 2000. J. Exp. Med. 191:375. (Block)
Hoshino A, et al. 2003. Eur. J. Immunol. 33:861. (IHC)
Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Block)
Zheng X, et al. 2010. Invest Ophthalmol Vis Sci. 51:3076. PubMed

RRID

AB_2860619 (BioLegend Cat. No. 108819) AB_2860620 (BioLegend Cat. No. 108820) AB_2860621 (BioLegend Cat. No. 108821) AB_2860622 (BioLegend Cat. No. 108822) AB_2860623 (BioLegend Cat. No. 108823) AB_2860624 (BioLegend Cat. No. 108824)

Antigen Details

Structure: NGF/TNF superfamily, 35 kD
Distribution: Activated B cells, antigen presenting cells
Function: B-cell/T-cell interaction, costimulation
Ligand/Receptor: OX40 (CD134)
Cell Type: Antigen-presenting cells, B cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Akiba H, et al. 1999. J. Immunol. 162:7058.
2. Stüber E, et al. 1995. Immunity 2:507.
3. Baum PR, et al. 1994. EMBO J. 13:3992.
Gene ID: 22164 View all products for this Gene ID
UniProt: View information about CD252 on UniProt.org

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