Alexa Fluor® 647 anti-mouse CD252 (OX40L) Anti-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1414	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Rat NRK-52E cell line transfected with mouse OX-40L

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

The RM134L antibody can block the costimulatory activity of OX40L. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections³, and in vivo and in vitro blocking of OX-40L-OX-40 functional interaction^1,2,4. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 108808).

Application References (PubMed link indicates BioLegend citation)

Akiba H, et al. 1999. J. Immunol. 162:7058. (Block)
Akiba H, et al. 2000. J. Exp. Med. 191:375. (Block)
Hoshino A, et al. 2003. Eur. J. Immunol. 33:861. (IHC)
Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Block)
Zheng X, et al. 2010. Invest Ophthalmol Vis Sci. 51:3076. PubMed

Product Citations

Elayeb R, et al. 2016. Haematologica. 101: 209 - 218. PubMed

RRID

AB_2256388 (BioLegend Cat. No. 108809) AB_2207379 (BioLegend Cat. No. 108810)

Antigen Details

Structure: NGF/TNF superfamily, 35 kD
Distribution: Activated B cells, antigen presenting cells
Function: B-cell/T-cell interaction, costimulation
Ligand/Receptor: OX40 (CD134)
Cell Type: Antigen-presenting cells, B cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Akiba H, et al. 1999. J. Immunol. 162:7058.
2. Stüber E, et al. 1995. Immunity 2:507.
3. Baum PR, et al. 1994. EMBO J. 13:3992.
Gene ID: 22164 View all products for this Gene ID
UniProt: View information about CD252 on UniProt.org

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