TotalSeq™-B0558 anti-mouse CD55 (DAF) Antibo-上海雅吉生物科技有限公司

规格	价格	库存
10µg	￥ 5166	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Transfected cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA

Preparation

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

PG - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.

Additional Product Notes

TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

Cao Q, et al. 2012. Am J Physiol Cell Physiol. 302:1731. PubMed.

RRID

AB_2876465 (BioLegend Cat. No. 131817)

Antigen Details

Structure: 60-70 kd single chain glycoprotein. While human CD55 contains only one CD55 gene, two distinct CD55 genes have been identified in the mouse, referred to as GPI-DAF and transmembrane(TM)-DAF. GPI-DAF is expressed only in testis.
Distribution: Red blood cells and most of the splenocytes, including B cells and T cells, and also many non-hematopoetic cells.
Function: Protect from complement attack by inhibition the deposition of C3 on antibody-sensitized cells.
Cell Type: B cells, Erythrocytes, T cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Miwa T et al. 2001. Immunology 104:207
2. Lin FY et al. 2001 Immunology 104:215
Gene ID: 13136 View all products for this Gene ID
UniProt: View information about CD55 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们