Alexa Fluor® 647 anti-mouse CD55 (DAF) Antibod
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD55 (DAF) Antibod
- 产品类别：
- 抗体
- 产品编号：
- 131805
- 产品应用：
- 131805
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Cao Q, et al. 2012. Am J Physiol Cell Physiol. 302:1731. PubMed.
- Product Citations
-
- Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
- Wilfahrt D, et al. 2021. Elife. 10:. PubMed
- Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
- Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed
- Inoue N, et al. 2020. Development. . PubMed
- RRID
- AB_1279263 (BioLegend Cat. No. 131805) AB_1279261 (BioLegend Cat. No. 131806)
- Structure
- 60-70 kd single chain glycoprotein. While human CD55 contains only one CD55 gene, two distinct CD55 genes have been identified in the mouse, referred to as GPI-DAF and transmembrane(TM)-DAF. GPI-DAF is expressed only in testis.
- Distribution
-
Red blood cells and most of the splenocytes, including B cells and T cells, and also many non-hematopoetic cells.
- Function
- Protect from complement attack by inhibition the deposition of C3 on antibody-sensitized cells.
- Cell Type
- B cells, Erythrocytes, T cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Miwa T et al. 2001. Immunology 104:207
2. Lin FY et al. 2001 Immunology 104:215
- Gene ID
- 13136 View all products for this Gene ID
- UniProt
- View information about CD55 on UniProt.org
