Ultra-LEAF™ Purified anti-human CD253 (Trail
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD253 (Trail
- 产品类别：
- 抗体
- 产品编号：
- 308213
- 产品应用：
- 308213
- Verified Reactivity
- Human
- Reported Reactivity
- African Green
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human TRAIL-transfected mouse cell line
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking1-4 of TRAIL-induced apoptosis and T cell mediated cytotoxicity. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 308214).
- Application References
(PubMed link indicates BioLegend citation) -
- Kayagaki N, et al. 1999. J. Immunol. 162:2639. (Block)
- Uno K, et al. 2003. Blood 101:3658. (Block)
- Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
- Denny MF, et al. 2007. Blood 110:2907. (Block)
- Kemter E, et al. 2011. Xenotransplantation. 19:40. PubMed
- RRID
- AB_2814154 (BioLegend Cat. No. 308213) AB_2814155 (BioLegend Cat. No. 308214)
- Structure
- TNF ligand superfamily member, approximately 30 kD
- Distribution
-
Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells
- Function
- Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity, TRAIL ligand of cognate receptors induces NF-κB signaling in a variety of cell types
- Ligand/Receptor
- DR4 and DR5 cognate receptors, also binds to the decoy receptors DcR1 and DcR2
- Cell Type
- Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Pitti RM, et al. 1996. J. Biol. Chem. 271:12687.
2. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.
3. Dorothee G, et al. 2002. J. Immunol. 169:809.
4. Crist SA, et al. 2004. Exp. Hematology 32:1073.
- Gene ID
- 8743 View all products for this Gene ID
- UniProt
- View information about CD253 on UniProt.org
