PE anti-human CD253 (TRAIL) Antibody
- 产品名称：
- PE anti-human CD253 (TRAIL) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 308205
- 产品应用：
- 308205
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1806 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- African Green
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human TRAIL-transfected mouse cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: blocking1-4 of TRAIL-induced apoptosis and T cell mediated cytotoxicity. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 308214).
- Application References
(PubMed link indicates BioLegend citation) -
- Kayagaki N, et al. 1999. J. Immunol. 162:2639. (Block)
- Uno K, et al. 2003. Blood 101:3658. (Block)
- Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
- Denny MF, et al. 2007. Blood 110:2907. (Block)
- Kemter E, et al. 2011. Xenotransplantation. 19:40. PubMed
- Product Citations
-
- Golinelli G, et al. 2021. Transl Oncol. 15:101240. PubMed
- Kalb M, et al. 2012. J Immunol. 188:1583. PubMed
- Kn?rck A, et al. 2022. Front Immunol. 13:838484. PubMed
- Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
- Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed
- Alonso R, et al. 2022. Blood Adv. :. PubMed
- Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
- Volpin V, et al. 2020. Cancer Immunol Res. 8:1163. PubMed
- Ray JE, et al. 2021. Cancer Biol Ther. 22:554. PubMed
- Lulla AR, et al. 2022. Am J Cancer Res. 12:315. PubMed
- Choi Y, et al. 2015. Gut. 64: 1303-1313. PubMed
- Achard C, et al. 2017. Oncoimmunology. 6:e1261240. PubMed
- RRID
- AB_345291 (BioLegend Cat. No. 308205) AB_2205825 (BioLegend Cat. No. 308206)
- Structure
- TNF ligand superfamily member, approximately 30 kD
- Distribution
-
Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells
- Function
- Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity, TRAIL ligand of cognate receptors induces NF-κB signaling in a variety of cell types
- Ligand/Receptor
- DR4 and DR5 cognate receptors, also binds to the decoy receptors DcR1 and DcR2
- Cell Type
- Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Pitti RM, et al. 1996. J. Biol. Chem. 271:12687.
2. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.
3. Dorothee G, et al. 2002. J. Immunol. 169:809.
4. Crist SA, et al. 2004. Exp. Hematology 32:1073.
- Gene ID
- 8743 View all products for this Gene ID
- UniProt
- View information about CD253 on UniProt.org
