Ultra-LEAF™ Purified anti-mouse CD71 Antibod
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD71 Antibod
- 产品类别：
- 抗体
- 产品编号：
- 113821
- 产品应用：
- 113821
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of cellular proliferation. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 113821 & 113822).
This clone may also be known as R17217 or R17 217.
- Application References
(PubMed link indicates BioLegend citation) -
- Trowbridge I, et al. 1982. J. Cell. Physiol. 112:403.
- Grisendi S, et al. 2005. Nature 437:147.
- van Rooy I, et al. 2010. J. Control Release 150:30. PubMed
- Willhelm BT, et al. 2011. Blood 117:27. PubMed
- Okasi Y, et al. 2012. Exp Hematol. 40:143PubMed
- Stojanov K, et al. 2012. Mol Pharm. 9:1620. PubMed
- RRID
- AB_2734171 (BioLegend Cat. No. 113821) AB_2734172 (BioLegend Cat. No. 113822)
- Structure
- Transferrin receptor and is 95 kD.
- Distribution
-
Most dividing cells, the brain endothelium, and cycling hematopoietic progenitors.
- Function
- Mediates iron uptake.
- Ligand/Receptor
- Transferrin.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Hentze MW, et al. 1996. P. Natl. Acad. Sci. USA 93:8175.
2. Trowbridge IS, et al. 1993. Annu. Rev. Cell Biol. 9:129.
3. Trowbridge I, et al. 1982. J. Cell Physiol. 112:403.
4. Kuhn LC, et al. 1984. Cell 37:95.
- Gene ID
- 22042 View all products for this Gene ID
- UniProt
- View information about CD71 on UniProt.org
