热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
PE anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody
产品名称：
PE anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody
产品类别：
抗体
产品编号：
108407
产品应用：
108407
[价格]
规格 价格 库存
50µg ￥ 784 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.

Special Note:?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 108436).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
  2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
  3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
  4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
  5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
  6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
  8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
  9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
  10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
  11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
  12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
  13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
  14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
  15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
  16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
  17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
  18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
  19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
  23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
  24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
  26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
  30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
  31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
  32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
  34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
  35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
Product Citations
  1. Ostapoff K, et al. 2014. Cancer Res. 74:4966. PubMed
  2. Drobek A, et al. 2015. J Immunol. 195: 3416 - 3426. PubMed
  3. Chinta KC et al. 2018. Cell reports. 25(7):1938-1952 . PubMed
  4. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  5. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  6. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  7. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
  8. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  9. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  10. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  11. Paschall A, et al. 2015. J Immunol. 194: 2369-2379. PubMed
  12. Su N, Xiong Y 2016. Sci Rep. 6:22658. PubMed
  13. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  14. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  15. Song C, et al. 2019. Nat Commun. 10:3745. PubMed
  16. Voronov E, et al. 2010. Int Immunol. 1.086805556. PubMed
  17. Matsushita Y, et al. 2021. J Bone Miner Res. 36:1145. PubMed
  18. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  19. Hu J, et al. 2021. Sci Adv. 7:. PubMed
  20. Shen H, et al. 2015. Infect Immun . 83: 2694 - 2704. PubMed
  21. Feng M, et al. 2014. Int Immunopharmacol. 19:267. PubMed
  22. Liu X, et al. 2013. Diabetes. 62:4228. PubMed
  23. Lee Y, et al. 2017. PLoS One.. 10.1371/journal.pone.0183028. PubMed
  24. Mirshahi F, et al. 2022. Cell Rep. 38:110454. PubMed
  25. Martin R, et al. 2014. J Leukoc Biol. 96:151. PubMed
  26. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  27. Peng J, et al. 2016. Nat Commun. 7:12029. PubMed
  28. Pan H, et al. 2020. Mol Psychiatry. . PubMed
  29. Brunner JS, et al. 2020. Nat Commun. 0.757638889. PubMed
  30. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  31. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  32. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  33. Somerville TD, et al. 2020. eLife. 9:e53381.. PubMed
  34. Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed
  35. Crnej A, et al. 2015. Invest Ophthalmol Vis Sci . 56:185. PubMed
  36. Etxeberria I, et al. 2020. Cancer Cell. 36(6):613-629. PubMed
  37. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  38. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  39. Kan C, et al. 2022. Bone Res. 10:62. PubMed
  40. Balachandran Y, et al. 2022. J Innate Immun. :1. PubMed
  41. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  42. Gordon RA, et al. 2020. PLoS One. 15:e0226396. PubMed
  43. Hawila E, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.104. PubMed
  44. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  45. Li J, et al. 2019. JCI Insight. 5. PubMed
  46. Casey S, et al. 2011. Mol Endocrinol. 25:933. PubMed
  47. Keshavarz M, et al. 2020. Virol J. 17:64. PubMed
  48. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  49. Canton J, et al. 2021. Nat Immunol. 22:140. PubMed
  50. Aebischer D, et al. 2014. PLoS One. 9:99297. PubMed
  51. Nayak RR, et al. 2021. Cell Host Microbe. 29(3):362-377.e11. PubMed
  52. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  53. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  54. H?nes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  55. Curtis MW, et al. 2018. Infect Immun. 86:e00186. PubMed
  56. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  57. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  58. Stumpfova M, et al. 2010. Cancer Res . 70:2962. PubMed
  59. Dang Y, et al. 2021. J Cancer. 12:7158. PubMed
  60. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  61. Yoon Y, et al. 2016. Stem Cell Reports. 7:840-853. PubMed
  62. Lira R, et al. 2016. PLoS One. 11: 0157475. PubMed
  63. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
  64. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  65. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  66. Yang M, et al. 2021. Cancer Immunol Immunother. 70:2467. PubMed
  67. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  68. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  69. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  70. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  71. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  72. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  73. Yan W, et al. 2021. Front Med (Lausanne). 8:752223. PubMed
  74. Aktories P, et al. 2022. Cell Rep Methods. 2:100260. PubMed
  75. Pashover-Schallinger E, et al. 2012. FASEB J. 26:3891. PubMed
  76. Wang Q, et al. 2018. Int J Biol Sci. 14:147. PubMed
  77. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  78. Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
  79. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  80. Patankar Y, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L902 - L913. PubMed
  81. Merlo LM, et al. 2020. Clin Pathol. 13:2632010X20951812. PubMed
  82. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
  83. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  84. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  85. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  86. Ibrahim ML, et al. 2018. Cell Rep. 25:3036. PubMed
  87. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  88. Chen Y, et al. 2022. Life Sci. 306:120826. PubMed
  89. Yuan M, et al. 2022. Oxid Med Cell Longev. 2022:5479491. PubMed
  90. Ostapoff K, et al. 2013. Mol Cancer Ther. 12:1190. PubMed
  91. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  92. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  93. Roderick J, et al. 2014. Proc Natl Acad Sci U S A. 111:14436. PubMed
  94. Rashid O, et al. 2013. Surgery. 153:771. PubMed
  95. Mosa A, et al. 2009. Infect Immun. 77:2908. PubMed
  96. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  97. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
  98. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  99. Koide S, et al. 2022. iScience. 25:103603. PubMed
  100. Filina J, et al. 2014. Cell Signal. 26:2138. PubMed
  101. Weckbach LT, et al. 2019. J Exp Med. 216:350. PubMed
  102. Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed
  103. Siolas D, et al. 2021. Cell Reports. 36(8):109578. PubMed
  104. Aguilera TA, et al. 2020. Clin Cancer Res. 26:2972. PubMed
  105. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  106. Beyerle A, et al. 2011. J Control Release. 151:51. PubMed
  107. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  108. Hsia HC, et al. 2017. J Leukoc Biol. 101:1053. PubMed
  109. Jordan PM, et al. 2021. Pharmaceuticals (Basel). 14:. PubMed
  110. Darlyuk-Saadon I, et al. 2021. FEBS J. 288:3978. PubMed
  111. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  112. Chen J, et al. 2021. Oncol Lett. 21:409. PubMed
  113. Hegde V, et al. 2015. J Immunol. 194:5211. PubMed
  114. Rowe R, et al. 2016. J Exp Med. 213: 1497 - 1512. PubMed
  115. Evavold CL, et al. 2021. Cell. 184(17):4495-4511.e19. PubMed
  116. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  117. Zhang M, et al. 2019. Autophagy. 1.423611111. PubMed
  118. Franklin R, et al. 2022. Nat Commun. 13:2350. PubMed
  119. Xie Y, et al. 2020. Endocrinology. 161:00:00. PubMed
  120. Ramos-Sevillano E, et al. 2016. PLoS One. 12: 1005500. PubMed
  121. Weimershaus M, et al. 2012. J Immunol. 188:1789. PubMed
  122. Guarnerio J, et al. 2018. Nat Commun. 9:66. PubMed
  123. Moriyasu T, et al. 2018. PLoS Negl Trop Dis. 12:e0006197. PubMed
  124. Terlizzi M, et al. 2021. Cell Physiol Biochem. 55:539. PubMed
RRID
AB_313372 (BioLegend Cat. No. 108407) AB_313373 (BioLegend Cat. No. 108408)

Antigen Details

Structure
21-25 kD
Distribution

Granulocytes, monocytes

Cell Type
Granulocytes, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID
17067 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G Ly-6C on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线