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Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1)
产品名称：
Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1)
产品类别：
抗体
产品编号：
108429
产品应用：
108429
[价格]
规格 价格 库存
25µg ￥ 1316 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.

Special Note:?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 108436).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
  2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
  3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
  4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
  5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
  6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
  8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
  9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
  10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
  11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
  12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
  13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
  14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
  15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
  16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
  17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
  18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
  19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
  23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
  24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
  26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
  30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
  31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
  32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
  34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
  35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
Product Citations
  1. Hildebrand D, et al. 2013. J Immunol. 190:4812. PubMed
  2. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  3. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  4. Schneider C, et al. 2018. Cell. 174:271. PubMed
  5. Billroth-MacLurg, AC, et al. 2016. J Immunol. 197:2208-2218. PubMed
  6. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  7. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
  8. Dow L, et al. 2014. PLoS One. 9:95236. PubMed
  9. Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed
  10. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  11. Szafran BN, et al. 2021. Chemical Research in Toxicology. 34(6):1556-1571. PubMed
  12. Manicone A, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.02.008. PubMed
  13. Rosen D, et al. 2016. J Infect Dis. 213: 649 - 658. PubMed
  14. Taube C, et al. 2011. PLoS One. 6:e21799. PubMed
  15. Yan J, et al. 2013. Free Radic Biol Med. 23:5849. PubMed
  16. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  17. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  18. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  19. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  20. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  21. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  22. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  23. Kuwayama R, et al. 2022. Nat Commun. 13:3107. PubMed
  24. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
  25. Dye BR, et al. 2020. Biomaterials. 234:119757. PubMed
  26. Chavez JS, et al. 2022. Cells. 11:. PubMed
  27. Manríquez V, et al. 2021. Nat Commun. 12:4547. PubMed
  28. Aguado B, et al. 2015. Sci Rep. 5: 17566. PubMed
  29. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
RRID
AB_893559 (BioLegend Cat. No. 108429) AB_893556 (BioLegend Cat. No. 108430)

Antigen Details

Structure
21-25 kD
Distribution

Granulocytes, monocytes

Cell Type
Granulocytes, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID
17067 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G Ly-6C on UniProt.org

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