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Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody
产品名称：
Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody
产品类别：
抗体
产品编号：
108403
产品应用：
108403
[价格]
规格 价格 库存
50µg ￥ 658 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IP, IHC, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.

Special Note:?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 108436).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
  2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
  3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
  4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
  5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
  6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
  8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
  9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
  10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
  11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
  12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
  13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
  14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
  15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
  16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
  17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
  18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
  19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
  23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
  24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
  26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
  30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
  31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
  32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
  34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
  35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
Product Citations
  1. Ahmed AU, et al. 2017. J Immunol. 199(6):2128. PubMed
  2. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  3. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  4. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  5. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  6. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  7. Zhu C, et al. 2018. Sci Rep. 5.113194444. PubMed
  8. Patel MM, et al. 2018. J Am Heart Assoc. 7:e010690. PubMed
  9. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  10. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
  11. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  12. Liu M, et al. 2022. J Exp Med. 219:. PubMed
  13. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  14. Dubeykovskaya Z, et al. 2016. Nat Commun. 7:10517. PubMed
  15. Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
  16. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  17. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
  18. Moon H et al. 2017. Cell stem cell. 21(5):665-678 . PubMed
  19. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  20. Gutiérrez-Gutiérrez ?, et al. 2021. EMBO Rep. 22:e52905. PubMed
  21. Scalzo S, et al. 2022. Int J Nanomedicine. 17:2865. PubMed
  22. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  23. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  24. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  25. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  26. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  27. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  28. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  29. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  30. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  31. Mikami H, et al. 2020. Nat Commun. 11:1162. PubMed
  32. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  33. Wu HC, et al. 2021. Cancer Discov. Online ahead of print. PubMed
  34. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  35. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
  36. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
  37. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  38. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  39. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  40. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  41. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  42. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  43. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  44. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  45. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  46. K?chl R, et al. 2020. Elife. 9:00. PubMed
  47. Getts D, et al. 2008. J Exp Med. 205:2319. PubMed
  48. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  49. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  50. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  51. Luo W et al. 2018. Immunity. 48(2):313-326 . PubMed
  52. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  53. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  54. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  55. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  56. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  57. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  58. Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed
  59. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  60. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  61. Perez E, et al. 2020. J Immunol. 205:2489. PubMed
  62. Aktories P, et al. 2022. Cell Rep Methods. 2:100260. PubMed
  63. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  64. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  65. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  66. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  67. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  68. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  69. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  70. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  71. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  72. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
  73. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  74. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  75. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  76. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  77. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  78. Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
  79. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  80. Shin JW, et al. 2020. Cell Death Dis. 0.667361111. PubMed
  81. Sch?nberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  82. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  83. Roderick J, et al. 2014. Proc Natl Acad Sci U S A. 111:14436. PubMed
  84. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  85. Baja?a S, et al. 2022. iScience. 25:103732. PubMed
  86. Michel A, et al. 2013. J Immunol. 90:5534. PubMed
  87. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
  88. Zhang C, et al. 2018. Cell Res. 28:323. PubMed
  89. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  90. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  91. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  92. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  93. Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed
  94. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  95. S?wen P et al. 2018. eLife. 7 pii: e41258. PubMed
  96. Ito K et al. 2019. Cell Rep. 28(10):2480-2490 . PubMed
  97. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  98. Morganti C, et al. 2022. EMBO Rep. 23:e54262. PubMed
  99. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  100. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  101. Teng Y, et al. 2021. Mol Ther. 29:2424. PubMed
  102. Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
  103. Olariu V, et al. 2021. Cell Reports. 34(2):108622. PubMed
  104. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  105. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
RRID
AB_313368 (BioLegend Cat. No. 108403) AB_313369 (BioLegend Cat. No. 108404)

Antigen Details

Structure
21-25 kD
Distribution

Granulocytes, monocytes

Cell Type
Granulocytes, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID
17067 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G Ly-6C on UniProt.org

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