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APC/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibod
产品名称：
APC/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibod
产品类别：
抗体
产品编号：
108423
产品应用：
108423
[价格]
规格 价格 库存
25µg ￥ 1414 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.

Special Note:?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 108436).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
  2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
  3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
  4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
  5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
  6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
  8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
  9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
  10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
  11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
  12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
  13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
  14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
  15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
  16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
  17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
  18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
  19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
  23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
  24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
  26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
  30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
  31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
  32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
  34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
  35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
Product Citations
  1. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  2. Hatzi K, et al. 2019. Nat Immunol. 20:86. PubMed
  3. Jaitin DA, et al. 2020. Cell. 178(3):686-698.e14.. PubMed
  4. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  5. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  6. Gu H, et al. 2021. Immun Inflamm Dis. 9:1686. PubMed
  7. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  8. Liu J, et al. 2012. PLoS One. 7:e44044. PubMed
  9. Bhattacherjee A, et al. 2019. Commun Biol. 2:450. PubMed
  10. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  11. Mayers S, et al. 2019. BMC Biotechnol. 19:21. PubMed
  12. Qiu F, et al. 2022. J Cancer. 13:2893. PubMed
  13. Gabbita S, et al. 2015. PLoS One. 10: e0137305. PubMed
  14. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  15. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  16. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  17. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  18. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  19. Vettorazzi S, et al. 2015. Nat Commun. 6: 7796. PubMed
  20. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  21. Liu Z, et al. 2021. Immunity. 54(2):247-258.e7. PubMed
  22. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  23. Firas J, et al. 2014. Differentiation. 87:193. PubMed
  24. Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed
  25. Lin W, et al. 2019. EBioMedicine. 49:133. PubMed
  26. Yahagi A, et al. 2019. RMD Open. 5:e000853. PubMed
  27. Gagnon JD, et al. 2019. Cell Rep. 28:2169. PubMed
  28. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  29. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  30. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  31. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  32. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  33. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  34. Xu G, et al. 2022. Front Pharmacol. 13:896601. PubMed
  35. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  36. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  37. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  38. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  39. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  40. Jee JJ, et al. 2022. Nat Commun. 13:18. PubMed
  41. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  42. Chen S, et al. 2015. Cancer Res . 7: 519-531. PubMed
  43. Kong IY, et al. 2020. Cell Rep. 33:108290. PubMed
  44. Young K, et al. 2021. Cell Stem Cell. . PubMed
  45. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
  46. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  47. Deets KA, et al. 2021. Elife. 10:. PubMed
  48. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
RRID
AB_2137486 (BioLegend Cat. No. 108423) AB_2137485 (BioLegend Cat. No. 108424)

Antigen Details

Structure
21-25 kD
Distribution

Granulocytes, monocytes

Cell Type
Granulocytes, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID
17067 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G Ly-6C on UniProt.org

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